Journal of Medical Sciences

ORIGINAL ARTICLE
Year
: 2019  |  Volume : 39  |  Issue : 5  |  Page : 217--222

A monoclonal enzyme-linked immunoassay for the detection of botulinum neurotoxin type E


Der-Jiang Chiao1, Jiunn-Jye Wey1, Wen-Zhi Lin1, Shiao-Shek Tang1, Pei-Yi Tsui1, Rong-Hwa Shyu1, Jyh-Hwa Kau1, Chih-Heng Huang1, Chuan-Wang Li2, Cheng-Cheung Chen1, Cheng-Che Liu3 
1 National Defense Medical Center, Institute of Preventive Medicine, Taipei, Taiwan
2 National Defense Medical Center, Institute of Preventive Medicine; Department and Graduate Institute of Microbiology and Immunology, National Defense Medical Center, Taipei, Taiwan
3 National Defense Medical Center, Institute of Preventive Medicine; National Defense Medical Center, Graduate Institute of Physiology, Taipei, Taiwan

Correspondence Address:
Dr. Cheng-Che Liu
National Defense Medical Center, Graduate Institute of Physiology, Room 6105, 6F, No. 161, Sec. 6, Minchuan E. Road, Taipei 114
Taiwan

Background and Aim: Botulinum neurotoxin Type E (BoNT/E), one of the most lethal toxin known, is the common contamination in fishery products or fish consumption that causes foodborne botulism. It is necessary to establish a sensitive and specific method for the detection of BoNT/E because of its extremely low lethal dose. Methods: In this study, a practical enzyme-linked immunosorbent assay for BoNT/E detection was developed. The assay is based on the sandwich format using monoclonal antibodies of two distinct specificities. An affinity-purified anti-BoNT/E light chain Mab (1E1) is utilized to adsorb BoNT/E from solution, and the second anti-BoNT/E heavy chain C terminus Mab (5E1) conjugated with peroxidase is then used to form sandwich complexes, and peroxidase allows color development and measurement of optical density at 450 nm. Results: Standard curves were linear over the range of 5–100 ng/ml BoNT/E. The limit of detection was below 10 ng/ml in phosphate-buffered saline buffer. The developed BoNT/E assay also showed no cross-reaction to Type A neurotoxin (BoNT/A) and Type B neurotoxin (BoNT/B). Conclusion: Herein, a sensitive and accurate ELISA for BoNT/E detection was presented. It has the potential to utilize in vivo BoNT/E analysis and contamination monitoring.


How to cite this article:
Chiao DJ, Wey JJ, Lin WZ, Tang SS, Tsui PY, Shyu RH, Kau JH, Huang CH, Li CW, Chen CC, Liu CC. A monoclonal enzyme-linked immunoassay for the detection of botulinum neurotoxin type E.J Med Sci 2019;39:217-222


How to cite this URL:
Chiao DJ, Wey JJ, Lin WZ, Tang SS, Tsui PY, Shyu RH, Kau JH, Huang CH, Li CW, Chen CC, Liu CC. A monoclonal enzyme-linked immunoassay for the detection of botulinum neurotoxin type E. J Med Sci [serial online] 2019 [cited 2019 Nov 15 ];39:217-222
Available from: http://www.jmedscindmc.com/article.asp?issn=1011-4564;year=2019;volume=39;issue=5;spage=217;epage=222;aulast=Chiao;type=0