Close
  Indian J Med Microbiol
 

Figure 4. Valproic acid (VPA) treatment induces apoptosis in vitro. SAS cells were treated with various concentrations of VPA and stained with phycoerythrin -conjugated annexin V and amino-actinomycin D (7-AAD) at (a) 24 or (b) 48 h. The percentage of annexin V-positive (X-axis) and/or 7-AAD-positive (Y-axis) cells was determined by performing flow cytometry. The percentages of annexin V-positive cells are presented in the plots. Statistical significance was determined by comparisons between VPA-treated and vehicle control-treated groups (n = 3; *P < 0.05; **P < 0.01)

Figure 4. Valproic acid (VPA) treatment induces apoptosis <i>in vitro</i>. SAS cells were treated with various concentrations of VPA and stained with phycoerythrin -conjugated annexin V and amino-actinomycin D (7-AAD) at (a) 24 or (b) 48 h. The percentage of annexin V-positive (X-axis) and/or 7-AAD-positive (Y-axis) cells was determined by performing flow cytometry. The percentages of annexin V-positive cells are presented in the plots. Statistical significance was determined by comparisons between VPA-treated and vehicle control-treated groups (<i>n</i> = 3; *<i>P</i> < 0.05; **<i>P</i> < 0.01)