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  Indian J Med Microbiol
 

Figure 5. Valproic acid (VPA) treatment suppresses the phosphorylation of Akt and ERK in vitro. (a) SAS cells were treated with different VPA concentrations (0, 1, 2, and 4 mM) for 48 h; immunoblotting was carried out for endogenous total and phosphorylated Akt and ERK proteins in control-and VPA-treated SAS cells. (b) Akt phosphorylation significantly decreased in a dose-dependent manner. (n = 3; *P < 0.05; **P < 0.01; ***P < 0.001). (c) ERK phosphorylation significantly decreased in a dose-dependent manner, (n = 3; *P < 0.05; **P < 0.01; ***P < 0.001)

Figure 5. Valproic acid (VPA) treatment suppresses the phosphorylation of Akt and ERK <i>in vitro</i>. (a) SAS cells were treated with different VPA concentrations (0, 1, 2, and 4 mM) for 48 h; immunoblotting was carried out for endogenous total and phosphorylated Akt and ERK proteins in control-and VPA-treated SAS cells. (b) Akt phosphorylation significantly decreased in a dose-dependent manner. (<i>n</i> = 3; *<i>P</i> < 0.05; **<i>P</i> < 0.01; ***<i>P</i> < 0.001). (c) ERK phosphorylation significantly decreased in a dose-dependent manner, (<i>n</i> = 3; *<i>P</i> < 0.05; **<i>P</i> < 0.01; ***<i>P</i> < 0.001)