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  Indian J Med Microbiol
 

Figure 3: Scheme of monoclonal antibody-based sandwich enzyme-linked immunosorbent assay for botulinum neurotoxin Type E detection A dual monoclonal antibody-based enzyme immunoassay was developed in this study. The monoclonal antibodies 1E1 and 5B1 were selected as the capture and detection antibody individually. The chemiluminescence signals can be read at 450 nm when o-phenylenediamine substrate was catalyzed with horseradish peroxidase (labeled on monoclonal antibody 5B1) after botulinum neurotoxin Type E detection

Figure 3: Scheme of monoclonal antibody-based sandwich enzyme-linked immunosorbent assay for botulinum neurotoxin Type E detection A dual monoclonal antibody-based enzyme immunoassay was developed in this study. The monoclonal antibodies 1E1 and 5B1 were selected as the capture and detection antibody individually. The chemiluminescence signals can be read at 450 nm when o-phenylenediamine substrate was catalyzed with horseradish peroxidase (labeled on monoclonal antibody 5B1) after botulinum neurotoxin Type E detection