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  Indian J Med Microbiol
 

Figure 3: Localization of TvAtg3 in high-glucose medium and glucose-restricted cultivation. (a) Subcellular localization of TvAtg3 in high-glucose medium-and glucose-restricted-cultured cells. The distribution of TvAtg3 in high-glucose medium and glucose-restricted-cultivated cells (24 h) was detected using anti-TvAtg3 antibody followed by confocal microscopy analysis. Scale bar = 5 μm. NC: the negative control probed only with a secondary antibody. (b) Localization of TvAtg3 after autophagy inhibition by Wort. Glucose-restricted-cultivated cells (24 h) were treated with Wort (50 and 100 μM) for 3 h and the distribution of TvAtg3 was monitored compared with that of the DMSO-treated control (Ctrl)

Figure 3: Localization of TvAtg3 in high-glucose medium and glucose-restricted cultivation. (a) Subcellular localization of TvAtg3 in high-glucose medium-and glucose-restricted-cultured cells. The distribution of TvAtg3 in high-glucose medium and glucose-restricted-cultivated cells (24 h) was detected using anti-TvAtg3 antibody followed by confocal microscopy analysis. Scale bar = 5 μm. NC: the negative control probed only with a secondary antibody. (b) Localization of TvAtg3 after autophagy inhibition by Wort. Glucose-restricted-cultivated cells (24 h) were treated with Wort (50 and 100 μM) for 3 h and the distribution of TvAtg3 was monitored compared with that of the DMSO-treated control (Ctrl)