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  Indian J Med Microbiol
 

Figure 4: TvAtg3 is required for autophagy. (a) Knockdown of TvAtg3 expression by specific siRNAs. Glucose-restricted-cultured cells were transfected with siRNAs (si-TvAtg3 #1, #2 and #3) targeting TvAtg3 or with a non-targeting siRNA as a negative control (Ct). The expression levels of TvAtg3 and TvAtg8 were analyzed and quantified at 24 h post transfection. (b) The effect of TvAtg3 knockdown on the formation of autophagic vacuoles. TvAtg3-knockdown cells (si-TvAtg3 #3) were costained with the autophagic green fluorescent dye and Lysotracker Red, and the formation of autophagic vacuoles and lysosomes was detected compared with that in the control group (si-Ctrl). Each dot represents one cell. Quantitation of autophagic vacuoles in TvAtg3-knockdown cells compared with that in the control group. The average number of autophagic vacuoles was determined by counting the autophagic green fluorescent signals in cells in different microscopic fields (30 cells, 10 cells/group). **P < 0.01

Figure 4: TvAtg3 is required for autophagy. (a) Knockdown of <i>TvAtg3</i> expression by specific siRNAs. Glucose-restricted-cultured cells were transfected with siRNAs (si-TvAtg3 #1, #2 and #3) targeting <i>TvAtg3</i> or with a non-targeting siRNA as a negative control (Ct). The expression levels of TvAtg3 and TvAtg8 were analyzed and quantified at 24 h post transfection. (b) The effect of <i>TvAtg3</i> knockdown on the formation of autophagic vacuoles. <i>TvAtg3</i>-knockdown cells (si-TvAtg3 #3) were costained with the autophagic green fluorescent dye and Lysotracker Red, and the formation of autophagic vacuoles and lysosomes was detected compared with that in the control group (si-Ctrl). Each dot represents one cell. Quantitation of autophagic vacuoles in <i>TvAtg3</i>-knockdown cells compared with that in the control group. The average number of autophagic vacuoles was determined by counting the autophagic green fluorescent signals in cells in different microscopic fields (30 cells, 10 cells/group). **<i>P</i> < 0.01