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  Indian J Med Microbiol
 

Figure 1: Drosophila E protein negatively regulates polypoid and salivary gland development. (a) The schematic diagram of a pair of Drosophila salivary glands in the third instar larval stage. The inset shows enlarge of salivary gland structure. The salivary glands consist of three cell types, including secretory cells (salivary gland, marked by blue color), the imaginal ring cells (marked by green color), and duct cells (salivary duct, marked by magenta color). Note that salivary gland and salivary duct are polypoid while the imaginal ring cells are diploid. Therefore, the nuclei of imaginal ring cells are much smaller than gland and duct cells. (b and d) Salivary glands of eyg-Gal4 or ptc-Gal4. (c and e and f) The indicated transgenic lines under the control of eyg-Gal4 (c) or ptc-Gal4 (e and f). E protein (green) staining represents the overexpression of E protein (c) when compared to the control (b). DAPI (blue) marks DNA (d-f). (g) The quantification of nuclear size for (d-f). Fifteen nuclei of each genotype are analyzed. Arbitrary unit denotes arbitrary unit. Area of nuclei is measured in microns using NIH Image J. Mean ± standard error of the mean is shown. (h) Salivary glands containing E protein overexpressing clones (act-Gal4 > GFP + E protein, GFP positive, green) and stained with Ex-lacZ (red). Ex-lacZ is a nuclear lacZ reporter. Note that GFP-positive nucleus (E protein overexpression) is smaller than GFP-negative nuclei. (i) The quantification of nuclear number in salivary glands. The number of nuclei (visualized by DAPI staining) is analyzed from 10 salivary glands of ptc-Gal4 and ptc > E protein, respectively. Note that each image for nuclear number counting are merged z-stacks. Mean ± standard error of the mean is shown. The scale bar is 50 μm

Figure 1: <i>Drosophila</i> E protein negatively regulates polypoid and salivary gland development. (a) The schematic diagram of a pair of <i>Drosophila</i> salivary glands in the third instar larval stage. The inset shows enlarge of salivary gland structure. The salivary glands consist of three cell types, including secretory cells (salivary gland, marked by blue color), the imaginal ring cells (marked by green color), and duct cells (salivary duct, marked by magenta color). Note that salivary gland and salivary duct are polypoid while the imaginal ring cells are diploid. Therefore, the nuclei of imaginal ring cells are much smaller than gland and duct cells. (b and d) Salivary glands of <i>eyg-Gal4</i> or <i>ptc-Gal4</i>. (c and e and f) The indicated transgenic lines under the control of <i>eyg-Gal4</i> (c) or <i>ptc-Gal4</i> (e and f). E protein (green) staining represents the overexpression of E protein (c) when compared to the control (b). DAPI (blue) marks DNA (d-f). (g) The quantification of nuclear size for (d-f). Fifteen nuclei of each genotype are analyzed. Arbitrary unit denotes arbitrary unit. Area of nuclei is measured in microns using NIH Image J. Mean ± standard error of the mean is shown. (h) Salivary glands containing E protein overexpressing clones (<i>act-Gal4 > GFP + E protein</i>, GFP positive, green) and stained with <i>Ex-lacZ</i> (red). <i>Ex-lacZ</i> is a nuclear lacZ reporter. Note that GFP-positive nucleus (E protein overexpression) is smaller than GFP-negative nuclei. (i) The quantification of nuclear number in salivary glands. The number of nuclei (visualized by DAPI staining) is analyzed from 10 salivary glands of <i>ptc-Gal4</i> and ptc > <i>E protein</i>, respectively. Note that each image for nuclear number counting are merged z-stacks. Mean ± standard error of the mean is shown. The scale bar is 50 μm