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  Indian J Med Microbiol
 

Figure 4: E2f1 is essential for E protein-mediated endoreplication defects. (a) Salivary glands containing E protein overexpressing clones (act-GAL4 > GFP + E protein, GFP positive, green, a') and stained with E2f1 antibody (red, a”). (b-d) The salivary glands of ptc-Gal4 (b), E protein driven by ptc-Gal4 (c), and E protein driven by ptc-Gal4 in heterozygous E2f1 mutant background (d). Note that removing one copy of E2f1 can partially rescue the size of nuclei and salivary gland in ptc > E protein flies. (e) The quantification of nuclear size for (b-d). Fifteen nuclei from (b-d) are analyzed. Mean ± standard error of the mean is shown. The scale bar is 50 μm

Figure 4: E2f1 is essential for E protein-mediated endoreplication defects. (a) Salivary glands containing E protein overexpressing clones (<i>act-GAL4 > GFP + E protein</i>, GFP positive, green, a') and stained with E2f1 antibody (red, a”). (b-d) The salivary glands of <i>ptc-Gal4</i> (b), E protein driven by <i>ptc-Gal4</i> (c), and E protein driven by <i>ptc-Gal4</i> in heterozygous <i>E2f1</i> mutant background (d). Note that removing one copy of <i>E2f1</i> can partially rescue the size of nuclei and salivary gland in ptc > <i>E protein</i> flies. (e) The quantification of nuclear size for (b-d). Fifteen nuclei from (b-d) are analyzed. Mean ± standard error of the mean is shown. The scale bar is 50 μm