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  Indian J Med Microbiol
 

Figure 5: S phase regulators are affected in E protein-mediated endoreplication defects. (a) A graph comparing cyclin E, PCNA, E2f1, and Rbf1 mRNA levels in eyg-Gal4 and E protein-overexpressing salivary glands, as measured by quantitative reverse transcription polymerase chain reaction. Note that cyclin E and PCNA are increased in E protein-overexpressing glands. (b and c) The salivary glands of ptc-Gal4 and cyclin E transgene driven by ptc-Gal4, respectively. Similar to overexpression of E protein, overexpression of Cyclin E reduces the size of nuclei and salivary gland. (d) The quantification of nuclear size for (b and c). Fifteen nuclei from (b and c) are analyzed. Mean ± standard error of the mean is shown. The scale bar is 50 μm

Figure 5: S phase regulators are affected in E protein-mediated endoreplication defects. (a) A graph comparing <i>cyclin E</i>, <i>PCNA</i>, <i>E2f1</i>, and <i>Rbf1</i> mRNA levels in <i>eyg-Gal4</i> and E protein-overexpressing salivary glands, as measured by quantitative reverse transcription polymerase chain reaction. Note that <i>cyclin E</i> and <i>PCNA</i> are increased in E protein-overexpressing glands. (b and c) The salivary glands of <i>ptc-Gal4</i> and <i>cyclin E</i> transgene driven by <i>ptc-Gal4</i>, respectively. Similar to overexpression of E protein, overexpression of Cyclin E reduces the size of nuclei and salivary gland. (d) The quantification of nuclear size for (b and c). Fifteen nuclei from (b and c) are analyzed. Mean ± standard error of the mean is shown. The scale bar is 50 μm