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  Indian J Med Microbiol
 

Figure 3: Secretion of ZIKV viral glycoproteins from BacMos-ZIKV prM-E-transduced mosquito cells: (a) Dot blot analysis for the detection of secreted ZIKV viral glycoproteins. AP-61 mosquito cells were transduced using BacMos-ZIKV prM-E at an MOI of 5. Culture medium was harvested at the indicated days posttransduction; (b) Western blot analysis of ZIKV E glycoproteins. V (the positive control) shows culture supernatant from the ZIKV-infected Vero cells; (c) Western blot analysis of ZIKV prM glycoproteins. Culture supernatants from mock (m), ZIKV-infected Vero (i), and transduced cells (t) were harvested at 3 days posttransduction

Figure 3: Secretion of ZIKV viral glycoproteins from BacMos-ZIKV prM-E-transduced mosquito cells: (a) Dot blot analysis for the detection of secreted ZIKV viral glycoproteins. AP-61 mosquito cells were transduced using BacMos-ZIKV prM-E at an MOI of 5. Culture medium was harvested at the indicated days posttransduction; (b) Western blot analysis of ZIKV E glycoproteins. V (the positive control) shows culture supernatant from the ZIKV-infected Vero cells; (c) Western blot analysis of ZIKV prM glycoproteins. Culture supernatants from mock (m), ZIKV-infected Vero (i), and transduced cells (t) were harvested at 3 days posttransduction