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  Indian J Med Microbiol
 

Figure 5: Coomassie blue staining (reducing) and Western blot analysis (non-reducing) of secreted, partially purified ZIKV glycoproteins. AP-61 cells were transduced with BacMos-ZIKV prM-E at an MOI of 5. Culture medium was harvested, concentrated, and diafiltrated using TFF. The indicated fractions (orig, original medium; ft, flow through; con, concentration) were subjected to Western blot analysis to detect ZIKV E (Anti-E, 6B6C) or prM glycoproteins. Arrows on the right indicate dimer-E (E2), mono-E (E), and prM glycoproteins

Figure 5: Coomassie blue staining (reducing) and Western blot analysis (non-reducing) of secreted, partially purified ZIKV glycoproteins. AP-61 cells were transduced with BacMos-ZIKV prM-E at an MOI of 5. Culture medium was harvested, concentrated, and diafiltrated using TFF. The indicated fractions (orig, original medium; ft, flow through; con, concentration) were subjected to Western blot analysis to detect ZIKV E (Anti-E, 6B6C) or prM glycoproteins. Arrows on the right indicate dimer-E (E2), mono-E (E), and prM glycoproteins